Reporter

Part:BBa_K812133:Design

Designed by: Anna Mlynarczyk   Group: iGEM12_Evry   (2012-09-20)


sfGFP with kozak sequence for expression in Xenopus


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 19


Design Notes

Kozak is included, as it cannot be added by biobrick. This part is ready for direct injection into a fertilised Xenopus tropicalis egg.


Source

Original sequence from A. victoria mutated to emit green light

References